Multiple Pseudomonas species were also detected in tissue biopsy specimens. Array analysis detected microbial targets in 51% of all wound samples, with Acinetobacter baumannii being the most frequently detected species. A subset of samples was also processed via next-generation sequencing and metagenomic analysis. We employed a microarray capable of detecting all sequenced pathogens for interrogation of 124 wound samples from extremity injuries in combat-injured U.S. We therefore sought to achieve comprehensive detection of microbial populations in wounds using novel genomic technologies and bioinformatics analyses. © 2012 Wiley Periodicals, Inc.Combat wound healing and resolution are highly affected by the resident microbial flora. By making use of this approach, the overall test sensitivity was 100%, and the test specificity ranged from 80 to 100%. Statistically, positive effects observed by the William's test were considered negative, if the mean of all replicates of a sample did not exceed the calculated TH. A threshold (TH) value based on a pooled NC was then calculated to exclude false positive test results. The data were first subjected to powerful analysis of variance (ANOVA) after an arcsine-square-root transformation to detect significant differences between the test samples and the negative control (NC). A two-step method for statistical evaluation of the test results is proposed and assessed in terms of specificity and sensitivity. Validity criteria (acceptable spontaneous and positive control-induced mutation counts) were fulfilled by 92–100%, depending on the test conditions. The laboratories tested four water samples (spiked/nonspiked) and two chemical mixtures with and without supplementation of a S9-mix.

Such a round-robin study is a precondition for both the finalization of the ISO standardization process and a possible regulatory implementation in water legislation. An international round-robin study on the Ames fluctuation test, a microplate version of the classic plate-incorporation method for the detection of mutagenicity in water, wastewater and chemicals was performed by 18 laboratories from seven countries.

Elster online download 2012